Yazama F, Sawada H, Hirosawa K, Hayashi Y, Nishida T
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Univ Tokyo, Japan.
Tissue Cell. 1991;23(2):235-46. doi: 10.1016/0040-8166(91)90078-8.
The Sertoli cell (blood-testis) barrier in the boar was visualized by the freeze-fracture, deep-etch, rotary-replication technique. Three kinds of cross-bridging structures were clearly recognized in the following three ectoplasmic specialization (ES) regions; (1) cross-bridges in the intercellular space between adjacent Sertoli cell membranes; (2) cross-bridges in the space between the Sertoli cell membrane and microfilament bundles; and (3) cross-bridges in the space between microfilament bundles and subsurface cisternae. Results from immunolocalization, vinculin and alpha-actinin were recognized in the Sertoli cell barrier. Our findings show that these structural elements of the Sertoli cell barrier are held together by these cross-bridging structures, and provide important morphological evidence that implicates the ES in the dynamic function of the microfilament bundles of the Sertoli cell barrier.
通过冷冻断裂、深度蚀刻、旋转复型技术观察了公猪的支持细胞（血睾）屏障。在以下三个外质特化（ES）区域清晰地识别出三种跨桥结构：（1）相邻支持细胞膜之间细胞间隙中的跨桥；（2）支持细胞膜与微丝束之间空间中的跨桥；（3）微丝束与表面下池之间空间中的跨桥。免疫定位结果显示，在支持细胞屏障中识别出了纽蛋白和α - 辅肌动蛋白。我们的研究结果表明，支持细胞屏障的这些结构元件通过这些跨桥结构结合在一起，并提供了重要的形态学证据，表明外质特化与支持细胞屏障微丝束的动态功能有关。
