Prestegarden Lars, Misra Anjan, Ware Marcus L, Yeh Ru-Fang, Bjerkvig Rolf, Feuerstein Burt G
NorLux Neuro Oncology, Department of Biomedicine, University of Bergen, Bergen, Norway.
Biotechniques. 2008 Jun;44(7):Piii-Pvi. doi: 10.2144/000112760.
Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.
阵列比较基因组杂交（aCGH）是一种强大的工具，用于检测相对DNA拷贝数，其分辨率仅受用于打印基因组阵列的细菌人工染色体（BAC）覆盖范围的限制。进行可靠的aCGH分析所需的DNA量一直是一个限制因素，尤其是在DNA有限的微小组织样本上。在这里，我们报告一种简单、高度灵敏且可靠的aCGH方法，用于分析不超过1 ng基因组DNA的样本。该技术的速度和简便性非常适合对针吸活检等小型临床样本进行研究。
