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利用近红外光谱法快速估算固态发酵物的单细胞油含量

Rapid estimation of single cell oil content of solid-state fermented mass using near-infrared spectroscopy.

作者信息

Peng Xiaowei, Chen Hongzhang

机构信息

Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.

出版信息

Bioresour Technol. 2008 Dec;99(18):8869-72. doi: 10.1016/j.biortech.2008.04.055. Epub 2008 Jun 4.

DOI:10.1016/j.biortech.2008.04.055
PMID:18534844
Abstract

Calibration model using near-infrared reflectance spectroscopy (NIRS) for estimation of SCO content in solid-state fermented mass was established. The NIRS calibration model was derived by partial least-squares (PLS) regression and prediction of SCO contents of independent solid-state fermented mass samples fermented by different oleaginous fungi showed the model to be rapid and accurate, giving R(2)-value higher than 0.9552 and root mean standard error of prediction (RMSEP) value lower than 0.5772%. The established NIRS calibration model could be used to estimate the SCO contents of the solid-state fermented masses and will provide much convenience to the research of SCO production in solid-state fermentation.

摘要

建立了利用近红外反射光谱(NIRS)估算固态发酵物料中SCO含量的校准模型。通过偏最小二乘法(PLS)回归得出NIRS校准模型,对不同产油真菌发酵的独立固态发酵物料样品的SCO含量进行预测,结果表明该模型快速且准确,决定系数(R²)值高于0.9552,预测均方根误差(RMSEP)值低于0.5772%。所建立的NIRS校准模型可用于估算固态发酵物料中的SCO含量,将为固态发酵中SCO生产的研究提供很大便利。

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