Srivastava A, Raj S K
Molecular Virology Lab, National Botanical Research Institute, Lucknow 226 001, India.
J Biosci. 2008 Jun;33(2):249-57. doi: 10.1007/s12038-008-0042-7.
Coat protein (CP) -mediated resistance against an Indian isolate of the Cucumber mosaic virus (CMV) subgroup IB was demonstrated in transgenic lines of Nicotiana benthamiana through Agrobacterium tumefaciens-mediated transformation. Out of the fourteen independently transformed lines developed, two lines were tested for resistance against CMV by challenge inoculations. The transgenic lines exhibiting complete resistance remained symptomless throughout life and showed reduced or no virus accumulation in their systemic leaves after virus challenge. These lines also showed virus resistance against two closely related strains of CMV. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IB member isolated from India.
通过根癌农杆菌介导的转化，在本氏烟草的转基因株系中证实了外壳蛋白（CP）介导的对黄瓜花叶病毒（CMV）亚组IB印度分离株的抗性。在培育出的14个独立转化株系中，通过挑战接种对其中两个株系进行了CMV抗性测试。表现出完全抗性的转基因株系在整个生命周期中都没有症状，并且在病毒挑战后其系统叶中的病毒积累减少或没有积累。这些株系还表现出对两种密切相关的CMV株系的病毒抗性。这是关于CP介导的对从印度分离的CMV亚组IB成员的转基因抗性的首次报道。
