Karasu Edibe, Sadan Gülay, Tasatargil Arda
Department of Pharmacology, Akdeniz University Medical Faculty, Antalya, Turkey.
Dig Dis Sci. 2008 Aug;53(8):2106-12. doi: 10.1007/s10620-008-0318-7. Epub 2008 Jun 6.
The effect of hyperhomocysteinemia induced by pretreatment with methionine 12 weeks prior to the study on the responses induced by gamma-aminobutyric acid (GABA), electrical field stimulation (EFS), and ATP have been evaluated in isolated rat duodenum. In the presence of adrenergic and cholinergic blockade, EFS (60 V, 1 ms, 1-3 Hz) induced frequency-dependent relaxations of the preparation. GABA and ATP also caused submaximal relaxation of the rat duodenum. The relaxations induced by GABA, EFS, and ATP were not significantly changed in duodenal tissues from hyperhomocysteinemic rats compared with control rats. GABA- and EFS-induced relaxations were inhibited by N-nitro-L-arginine methyl ester (L-NAME; 3 x 10(-4) M) in both hyperhomocysteinemic and control rats. On the other hand, L-NAME incubation did not affect ATP-induced relaxation. These results suggest that hyperhomocysteinemia does not cause an important impairment on non-adrenergic non-cholinergic innervation of the rat duodenum.
在研究前12周用蛋氨酸预处理诱导高同型半胱氨酸血症对γ-氨基丁酸（GABA）、电场刺激（EFS）和ATP在离体大鼠十二指肠中诱导的反应的影响已进行了评估。在肾上腺素能和胆碱能阻断的情况下，EFS（60 V，1 ms，1 - 3 Hz）诱导标本出现频率依赖性舒张。GABA和ATP也引起大鼠十二指肠的次最大舒张。与对照大鼠相比，高同型半胱氨酸血症大鼠十二指肠组织中GABA、EFS和ATP诱导的舒张没有明显变化。在高同型半胱氨酸血症大鼠和对照大鼠中，GABA和EFS诱导的舒张均被N-硝基-L-精氨酸甲酯（L-NAME；3×10⁻⁴ M）抑制。另一方面，L-NAME孵育不影响ATP诱导的舒张。这些结果表明，高同型半胱氨酸血症不会对大鼠十二指肠的非肾上腺素能非胆碱能神经支配造成重要损害。
