Perfluorohexane-loaded macrophages as a novel ultrasound contrast agent: a feasibility study.
作者信息
Kornmann Liselotte M, Curfs Daniëlle M J, Hermeling Evelien, van der Made Ingeborg, de Winther Menno P J, Reneman Robert S, Reesink Koen D, Hoeks Arnold P G
机构信息
Department of Biophysics, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, P. O. Box 616, 6200 MD, Maastricht, The Netherlands.
出版信息
Mol Imaging Biol. 2008 Sep;10(5):264-70. doi: 10.1007/s11307-008-0146-3. Epub 2008 Jun 7.
PURPOSE
We investigated in vitro the potential of macrophages to act as targeted vehicle for ultrasound molecular imaging.
PROCEDURES
Murine bone marrow-derived macrophages (BMM), incubated for 3 h with different concentrations of perfluorohexane (PFH) emulsions, were monitored by microscopy, flow cytometry, and ultrasound. Effects of PFH loading on BMM adhesion molecule (PSGL-1, VLA-4, Mac-1, LFA-1) expression were analyzed by flow cytometry. Static adhesion of PFH loaded BMM to unstimulated and TNF-alpha stimulated b.End5 endothelial cells was assessed by microscopy.
RESULTS
Incubation of BMM with PFH emulsions resulted in dose-dependent uptake and increased echogenicity (max. 17 dB). Flow cytometry analyses revealed no down-regulation related to PFH loading of BMM adhesion molecule expression. Endothelial adhesion remained functional, even after 24 h, although PFH loading dose-dependently attenuated static adhesion.
CONCLUSION
PFH loaded BMM may potentially serve as ultrasound contrast agent for noninvasive detection of atherogenic hotspots in arteries.
Zotero 插件