Liang Chuan-Dong, Li Wen-Ling, Liu Na, Yin Yu, Hao Jun, Zhao Wen-Qing
Neurosurgery Department, Hebei Medical University, Shijiazhuang 050000, China.
Neurosci Lett. 2008 Jul 18;439(3):250-5. doi: 10.1016/j.neulet.2008.05.046. Epub 2008 May 17.
To examine the effects of gamma knife surgery (GKS) on the expression of N-methel-D-asparate receptor (NMDAR) subunits in rat forebrain.
Using stereotactic technique, we performed gamma knife irradiation on the left forebrain of 13 male Wistar rats with a maximum dose of 60 Gy. These animals were raised for 24h, 30 and 60 days before they were killed. Then immunohistochemistry was applied to detect the relative levels of NMDAR subunits (NR1, NR2A, and NR2B) in the target region.
The expression of NR1 and NR2A but not NR2B increased significantly in the cortex 30 and 60 days after irradiation. However, no significant differences in the expression of these three subunits were detected in the caudate putamen at all time points.
gamma knife irradiation induced the upregulation of NMDAR subunits, NR1, and NR2A, which might represent a possible mechanism underlying the therapeutic effects of gamma knife irradiation on many neurological diseases, including drug resistance epilepsy.
研究伽玛刀手术（GKS）对大鼠前脑N-甲基-D-天冬氨酸受体（NMDAR）亚基表达的影响。
采用立体定向技术，对13只雄性Wistar大鼠的左前脑进行伽玛刀照射，最大剂量为60 Gy。这些动物在处死前分别饲养24小时、30天和60天。然后应用免疫组织化学方法检测靶区NMDAR亚基（NR1、NR2A和NR2B）的相对水平。
照射后30天和60天，皮质中NR1和NR2A的表达显著增加，而NR2B的表达无明显变化。然而，在所有时间点，尾状核中这三个亚基的表达均未检测到显著差异。
伽玛刀照射诱导了NMDAR亚基NR1和NR2A的上调，这可能是伽玛刀照射对包括耐药性癫痫在内的许多神经系统疾病产生治疗作用的潜在机制。
