Do Tri, Ho Frank, Heidecker Bettina, Witte Krista, Chang Lucille, Lerner Laura
Raven Biotechnologies, Inc. Manufacturing, One Corporate Drive, South San Francisco, CA 94080, USA.
Protein Expr Purif. 2008 Aug;60(2):147-50. doi: 10.1016/j.pep.2008.04.009. Epub 2008 May 3.
Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins, in solution. The presence of other, non-binding molecules does not interfere with quantification, which allows one to measure the concentration of the molecule of interest in a crude mixture. Here we apply this method to determining the dynamic binding capacity of affinity resins.
生物层干涉术是一种用于定量溶液中大分子（如蛋白质）的新方法。其他非结合分子的存在不会干扰定量，这使得人们能够在粗混合物中测量目标分子的浓度。在这里，我们应用这种方法来测定亲和树脂的动态结合能力。
