Keiser Paul B, Coulibaly Yaya, Kubofcik Joseph, Diallo Abdallah A, Klion Amy D, Traoré Sekou F, Nutman Thomas B
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Mol Biochem Parasitol. 2008 Aug;160(2):123-8. doi: 10.1016/j.molbiopara.2008.04.012. Epub 2008 May 2.
Wolbachiae are bacterial endosymbionts of insects and many filarial nematodes whose products trigger inflammation in filarial infections. The dependence of the parasites on their endosymbionts has also led to the use of antibiotics directed against the Wolbachiae, therapy that has been demonstrated to have a profound salutary effect on filarial infections. The identification of Wolbachiae in Mansonella species has been conclusively shown for Mansonella ozzardi (Mo), but not for Mansonella perstans (Mp). Using primers known to amplify the 16S ribosomal DNA of other filarial Wolbachiae, an identical 1393bp band was found in all samples tested. Sequence analysis of these samples demonstrated a single consensus sequence for Mp Wolbachia 16S rDNA that was most similar to Wolbachia sequences from other filarial nematodes. When aligned with the only other Mansonella Wolbachia sequence (Mo) there were only 8 nucleotide differences in the 1369bp overlapping sequence. Phylogenetic dendrograms, examining the relationship of the Mp Wolbachia to other Wolbachia 16S rDNA, showed that the Wolbachia tracked almost identically to the 5S rRNA of their parasite host. Wolbachia surface protein (WSP) was also demonstrated in protein extracted from Mp-containing whole blood. In advance of a treatment trial of Mp, a method for the quantitation of Mp Wolbachia was developed and used to demonstrate not only a relationship between microfilarial numbers and Wolbachia copy numbers, but also to demonstrate the effect of antibiotic on ridding Mp of Wolbachia.
沃尔巴克氏体是昆虫和许多丝虫线虫的细菌内共生体，其产物在丝虫感染中引发炎症。寄生虫对其内共生体的依赖也导致了使用针对沃尔巴克氏体的抗生素进行治疗，已证明这种疗法对丝虫感染有深远的有益影响。已确凿地证实在奥氏曼森线虫（Mo）中存在沃尔巴克氏体，但在常现曼森线虫（Mp）中尚未证实。使用已知可扩增其他丝虫沃尔巴克氏体16S核糖体DNA的引物，在所有测试样本中均发现了一条相同的1393bp条带。对这些样本的序列分析表明，Mp沃尔巴克氏体16S rDNA有一个单一的共有序列，与其他丝虫线虫的沃尔巴克氏体序列最为相似。当与唯一的另一种曼森线虫沃尔巴克氏体序列（Mo）比对时，在1369bp的重叠序列中只有8个核苷酸差异。系统发育树状图研究了Mp沃尔巴克氏体与其他沃尔巴克氏体16S rDNA的关系，结果表明沃尔巴克氏体的追踪结果与其寄生虫宿主的5S rRNA几乎完全相同。在含Mp的全血提取物中也证实了沃尔巴克氏体表面蛋白（WSP）。在对Mp进行治疗试验之前，开发了一种定量Mp沃尔巴克氏体的方法，并用于证明不仅微丝蚴数量与沃尔巴克氏体拷贝数之间存在关系，还证明了抗生素清除Mp中沃尔巴克氏体的效果。
