Vandekerckhove Annelies, Glorieux Sarah, Broeck Wim Van den, Gryspeerdt Annick, van der Meulen Karen M, Nauwynck Hans J
Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Vet J. 2009 Sep;181(3):280-7. doi: 10.1016/j.tvjl.2008.03.027. Epub 2008 Jun 6.
An in vitro model of the upper respiratory tract of the horse was developed to investigate mechanisms of respiratory diseases. Four tissues of the upper respiratory tract of three horses were collected. Explants were maintained in culture at an air-liquid interface for 96h. At 0, 24, 48, 72 and 96h of cultivation, a morphometric analysis was performed using light microscopy, scanning electron microscopy and transmission electron microscopy. The explants were judged on morphometric changes of epithelium, basement membrane and connective tissue. Viability was evaluated using a fluorescent Terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labelling (TUNEL) staining. No significant changes in morphometry and viability of any of the explants were observed during cultivation. Hence, the in vitro model may be useful to study infectious and non-infectious diseases at the level of the equine respiratory tract, with potential application to the development of vaccines and treatments for diseases of the respiratory tract.
为了研究呼吸道疾病的发病机制，开发了一种马的上呼吸道体外模型。收集了三匹马的上呼吸道的四种组织。将外植体在气液界面培养96小时。在培养的0、24、48、72和96小时，使用光学显微镜、扫描电子显微镜和透射电子显微镜进行形态计量分析。根据上皮、基底膜和结缔组织的形态计量变化对外植体进行判断。使用荧光末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）染色评估活力。在培养过程中，未观察到任何外植体的形态计量和活力有显著变化。因此，该体外模型可能有助于在马呼吸道水平上研究感染性和非感染性疾病，在呼吸道疾病疫苗和治疗方法的开发中具有潜在应用价值。
