Kucharski J, Stefańczyk-Krzymowska S, Jana B
Division of Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
Pol J Vet Sci. 2008;11(1):9-16.
The aim of the present study was to estimate the absorption of 125I-labeled proinflammatory cytokines--interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) from inflamed porcine uterus into the uterine venous blood. Moreover, in order to test the hypothesis that the above cytokines penetrate directly into ovaries and oviduct via local destination transfer in the area of the ovarian vascular pedicle and bypassing the systemic circulation, the concentration of IL-1beta, IL-6 and TNF-alpha in ovarian and oviductal tissues was also studied. These cytokine concentrations were also estimated in the ovarian venous blood. IL-1beta, IL-6 and TNF-alpha from both control and inflamed uteri were absorbed into the uterine venous blood, but it was higher (P < 0.05-0.001) from the pathologically changed uteri. The uterine tissues, particularly the endometrium, of both control and inflamed uteri retained all studied cytokines, but to a higher degree (P < 0.001) in the inflamed uteri. Injections of IL-1beta, IL-6 and TNF-alpha into the control and inflammatory changed uteri produced the presence of these proteins in the ovary and oviduct. However, the concentrations of IL-1beta and IL-6 in the ovarian and oviductal tissues was low after injections of control and inflamed uteri with these cytokines. In turn, administration of TNF-alpha into the inflammatory changed uteri lead to an enhancement in the concentration of this cytokine in the ovarian parenchyma (P < 0.05) and oviduct (P < 0.001). All studied cytokines were found in the ovarian venous blood after their injection into both control and inflamed uteri, which indicated its local destination transfer to the ovary. However, the concentration of cytokines increased (P <0.05-0.001) in the gilts with pathologically changed uteri as compared to controls. The study showed that both control and inflamed porcine uteri absorbed IL-1beta, IL-6 and TNF-alpha into the uterine venous blood, but the values of absorbed cytokines from inflamed uteri were higher. Moreover, the quantity and the manner of the studied cytokineS absorption into the uterine venous blood differed.
本研究的目的是评估125I标记的促炎细胞因子——白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）从发炎的猪子宫吸收进入子宫静脉血的情况。此外，为了验证上述细胞因子通过卵巢血管蒂区域的局部靶向转运直接进入卵巢和输卵管并绕过体循环这一假设，还研究了卵巢和输卵管组织中IL-1β、IL-6和TNF-α的浓度。这些细胞因子的浓度也在卵巢静脉血中进行了评估。来自对照子宫和发炎子宫的IL-1β、IL-6和TNF-α均被吸收进入子宫静脉血，但来自病理改变子宫的吸收量更高（P<0.05 - 0.001）。对照子宫和发炎子宫的子宫组织，特别是子宫内膜，均保留了所有研究的细胞因子，但发炎子宫中的保留程度更高（P<0.001）。向对照子宫和炎症改变的子宫注射IL-1β、IL-6和TNF-α后，在卵巢和输卵管中检测到了这些蛋白质。然而，用这些细胞因子注射对照子宫和发炎子宫后，卵巢和输卵管组织中IL-1β和IL-6的浓度较低。相反，向炎症改变的子宫注射TNF-α导致该细胞因子在卵巢实质（P<0.05）和输卵管（P<0.001）中的浓度升高。将所有研究的细胞因子注射到对照子宫和发炎子宫后，在卵巢静脉血中均检测到了这些细胞因子，这表明其向卵巢的局部靶向转运。然而，与对照组相比，病理改变子宫的后备母猪中细胞因子的浓度升高（P<0.05 - 0.001）。研究表明，对照子宫和发炎的猪子宫均将IL-1β、IL-6和TNF-α吸收进入子宫静脉血，但来自发炎子宫的吸收细胞因子的值更高。此外，所研究的细胞因子吸收进入子宫静脉血的量和方式有所不同。
