Jung Chan-Kwon, Lee Ahwon, Jung Eun-Sun, Choi Yeong-Jin, Jung So-Lyung, Lee Kyo-Young
Department of Hospital Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Acta Cytol. 2008 May-Jun;52(3):313-9. doi: 10.1159/000325513.
To compare the efficacy of the SurePath (SP) vs. conventional smears (CS) in fine needle aspiration (FNA) of thyroid gland lesions.
A total of 193 FNA cases with thyroid nodules were studied. Samples from ultrasound-guided FNA were split to prepare CS and SP slides. The diagnostic categories of unsatisfactory, benign, atypical and malignant were compared. Galectin-3 immunostaining was performed on SP slides.
Some differences were found between the cytomorphology of CS and SP. SP slides showed more increased cellularity and more clustered tissue fragments. On SP slides, nuclear detail and nucleoli were more easily detected and nuclear irregularity was very useful for the diagnosis of papillary carcinoma. SP showed a trend toward a lower proportion of atypical category. The overall sensitivity of FNA in diagnosing thyroid neoplasm was 90.9% for CS and 93.9% for SP. Most lesions (73%) diagnosed as papillary carcinoma after surgery showed positive staining of galectin-3.
The SP method showed easy evaluation of cytomorphologic features and consistent specimen quality and appeared to be more useful in diagnosing the suspicious cases. Moreover, it offered the possibility of adjunctive immunocytochemistry on the same sample.
比较SurePath（SP）法与传统涂片（CS）法在甲状腺病变细针穿刺活检（FNA）中的效果。
共研究了193例甲状腺结节FNA病例。超声引导下FNA获取的样本分成两份，分别制备CS涂片和SP涂片。比较不满意、良性、非典型性和恶性等诊断类别。对SP涂片进行半乳凝素-3免疫染色。
CS和SP的细胞形态学存在一些差异。SP涂片显示细胞增多更明显，组织碎片更聚集。在SP涂片上，核细节和核仁更容易观察到，核不规则对乳头状癌的诊断非常有用。SP显示非典型性类别比例有降低趋势。FNA诊断甲状腺肿瘤的总体敏感性，CS为90.9%，SP为93.9%。术后诊断为乳头状癌的大多数病变（73%）半乳凝素-3染色呈阳性。
SP法对细胞形态学特征评估容易，标本质量一致，在诊断可疑病例中似乎更有用。此外，它还提供了对同一样本进行辅助免疫细胞化学检测的可能性。
