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在Flt3配体和白细胞介素-6存在的情况下长期培养后，人脐带血CD34造血干细胞和祖细胞的基质细胞衍生因子-1α依赖性迁移从依赖蛋白激酶C（PKC）-α转变为不依赖PKC-α。

The stromal cell-derived factor-1alpha dependent migration of human cord blood CD34 haematopoietic stem and progenitor cells switches from protein kinase C (PKC)-alpha dependence to PKC-alpha independence upon prolonged culture in the presence of Flt3-ligand and interleukin-6.

作者信息

Kasenda Benjamin, Kassmer Susannah H, Niggemann Bernd, Schiermeier Sven, Hatzmann Wolfgang, Zänker Kurt S, Dittmar Thomas

机构信息

Institute of Immunology, Witten/Herdecke University, Witten, Germany.

出版信息

Br J Haematol. 2008 Sep;142(5):831-5. doi: 10.1111/j.1365-2141.2008.07256.x. Epub 2008 Jun 5.

DOI:10.1111/j.1365-2141.2008.07256.x
PMID:18540940
Abstract

Addition of the inflammatory cytokine interleukin (IL)-6 to the culture medium of human cord blood haematopoietic stem and progenitor cells (HSPCs) has been shown to lead to an altered stromal cell-derived factor-1alpha-dependent migratory phenotype. This study investigated whether this effect was attributed to a differential engagement of protein kinase C (PKC) isotypes. The migratory activity of both Flt3-ligand and Flt3-ligand/IL-6 cultured cord blood HSPCs was PKC-alpha dependent on day 1, but PKC-alpha independent after 5 d of cultivation. PKC-alpha expression was not down-regulated in cells cultured for 5 d indicating a switch of signalling molecules directing cell migration.

摘要

已证明，在人脐带血造血干细胞和祖细胞（HSPCs）的培养基中添加炎性细胞因子白细胞介素（IL）-6会导致基质细胞衍生因子-1α依赖性迁移表型发生改变。本研究调查了这种效应是否归因于蛋白激酶C（PKC）同工型的不同参与。在培养第1天，Flt3配体和Flt3配体/IL-6培养的脐带血HSPCs的迁移活性均依赖PKC-α，但培养5天后则不依赖PKC-α。在培养5天的细胞中，PKC-α表达未下调，表明指导细胞迁移的信号分子发生了转换。

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