McLean D M, Grass P N, Judd B D, Ligate L V, Peter K K
J Hyg (Lond). 1977 Aug;79(1):61-71. doi: 10.1017/s0022172400052852.
Strains of California encephalitis virus (snowshoe hare subtype) were isolated from 8 of 475 pools comprising 23 747 unengorged female mosquitoes of five species collected at three of six locations throughout the Mackenzie Valley of the Northwest Territories, Canada, from latitudes 60 to 69 degrees N between 10 and 24 July 1976. Minimum field infection rates included 1:2734 for Aedes communis, 1:256 to 1:3662 for A. hexodontus and 1:911 to 1:1611 for A. punctor. Northway virus was also isolated from 1 of 3662 A. hexodontus mosquitoes collected at Inuvik (69 degrees N, 135 degrees W). Transmission of CE virus by A. communis infected by feeding on virus in defibrinated blood and incubation at 0, 13 and 23 degrees C for 13-20 days clearly demonstrates the importance of this species as a natural vector, and transmission of CE virus by Culiseta inornata after incubation at 0 and 13 degrees C following intrathoracic injection strengthens evidence of its role as a natural vector. Immunofluorescence was less reliable than imunoperoxidase for detection of CE viral antigen in mosquito salivary glands.
1976年7月10日至24日期间，在加拿大西北地区麦肯齐山谷六个地点中的三个地点，从475组共23747只未吸血的五种雌性蚊子中采集样本，其中8组分离出加利福尼亚脑炎病毒（雪兔亚型）毒株。采集地点位于北纬60至69度之间。最低野外感染率包括：普通伊蚊为1:2734，六齿伊蚊为1:256至1:3662，刺扰伊蚊为1:911至1:1611。在因纽维克（北纬69度，西经135度）采集的3662只六齿伊蚊中，也分离出了诺思韦病毒。通过取食去纤维蛋白血感染病毒并在0、13和23摄氏度下孵育13 - 20天的普通伊蚊传播加利福尼亚脑炎病毒，清楚地证明了该物种作为自然传播媒介的重要性；胸腔内注射后在0和13摄氏度下孵育的无饰库蚊传播加利福尼亚脑炎病毒，进一步证明了其作为自然传播媒介的作用。在检测蚊子唾液腺中的加利福尼亚脑炎病毒抗原时，免疫荧光法不如免疫过氧化物酶法可靠。
