大肠杆菌中重组蛋白表达的自动化筛选

Automated recombinant protein expression screening in Escherichia coli.

作者信息

Busso Didier, Stierlé Matthieu, Thierry Jean-Claude, Moras Dino

机构信息

Structural Biology and Genomics Platform, IGBMC, CNRS/INSERM/Université Louis Pasteur, Illkirch, France.

出版信息

Methods Mol Biol. 2008;426:175-86. doi: 10.1007/978-1-60327-058-8_10.

DOI:10.1007/978-1-60327-058-8_10

PMID:18542863

Abstract

To fit the requirements of structural genomics programs, new as well as classical methods have been adapted to automation. This chapter describes the automated procedure developed within the Structural Biology and Genomics Platform, Strasbourg for performing recombinant protein expression screening in Escherichia coli. The procedure consists of parallel competent cells transformation, cell plating, and liquid culture inoculation, implemented for up to 96 samples at a time.

摘要

为满足结构基因组学计划的要求，新的以及经典的方法都已适用于自动化操作。本章描述了在斯特拉斯堡结构生物学与基因组学平台内开发的用于在大肠杆菌中进行重组蛋白表达筛选的自动化程序。该程序包括平行感受态细胞转化、细胞铺板和液体培养接种，一次可对多达96个样品进行操作。