Lesley Scott A
Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.
Methods Enzymol. 2009;463:767-85. doi: 10.1016/S0076-6879(09)63041-X.
Protein properties are highly diverse, making parallel expression and purification a particular challenge. Parallel methods are typically used when a number derivatives of a target protein are desired or when multiple homologs are needed. A typical scenario involves target evaluation, cloning and mutagenesis of the target, expression screening, large-scale expression and purification, and analytical and biophysical testing of the resulting protein. This chapter describes some of the strategies and methods employed for parallel protein expression and purification.
蛋白质特性高度多样，这使得并行表达和纯化成为一项特殊挑战。当需要目标蛋白质的多个衍生物或需要多个同源物时，通常会使用并行方法。一个典型的流程包括目标评估、目标的克隆和诱变、表达筛选、大规模表达和纯化，以及对所得蛋白质进行分析和生物物理测试。本章描述了用于并行蛋白质表达和纯化的一些策略和方法。
