利用交联、质谱分析和分子建模相结合的方法进行蛋白质结构测定。

Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling.

作者信息

Mouradov Dmitri, King Gordon, Ross Ian L, Forwood Jade K, Hume David A, Sinz Andrea, Martin Jennifer L, Kobe Bostjan, Huber Thomas

机构信息

School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia.

出版信息

Methods Mol Biol. 2008;426:459-74. doi: 10.1007/978-1-60327-058-8_31.

DOI:10.1007/978-1-60327-058-8_31

PMID:18542884

Abstract

Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.

摘要

交联结合质谱分析可作为蛋白质复合物和多结构域蛋白质结构建模的工具。尽管交联仅代表较弱的结构限制，但一组有限的实验交联与分子对接/建模相结合通常足以在低分辨率下确定蛋白质复合物或多结构域蛋白质的结构。