Farmer T B, Caprioli R M
Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt Unitversity School of Medicine, Nashville, Tennessee 37232-6400, USA.
J Mass Spectrom. 1998 Aug;33(8):697-704. doi: 10.1002/(SICI)1096-9888(199808)33:8<697::AID-JMS711>3.0.CO;2-H.
A number of different procedures have been developed for use with matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for the analysis of non-covalent protein-protein complexes. These include use of specific matrix and laser combinations, accumulation of "first shot" spectra, modification of pH and solvent conditions during sample preparation and use of cross-linking agents to attach the monomers covalently to each other in the complex. The results have shown the techniques to be effective with some but not all complexes, although cross-linking is the most successful. The physical and chemical nature of the complex is critical and therefore a diversity of approaches is recommended for such studies.
已经开发出多种不同的程序，用于与基质辅助激光解吸/电离质谱法（MALDI/MS）结合，以分析非共价蛋白质-蛋白质复合物。这些程序包括使用特定的基质和激光组合、积累“首次进样”光谱、在样品制备过程中改变pH值和溶剂条件，以及使用交联剂使复合物中的单体彼此共价连接。结果表明，这些技术对某些复合物有效，但并非对所有复合物都有效，尽管交联是最成功的方法。复合物的物理和化学性质至关重要，因此建议针对此类研究采用多种方法。
