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[对含有无机磷酸盐的腺嘌呤和二磷酸腺苷冷冻溶液在77K下近紫外辐射的自由基产物的分析]

[Analysis of free-radical products of near-UV irradiation at 77 K of frozen adenine and adenosine diphosphate solutions containing inorganic phosphate].

作者信息

Lozinova T A, Brzhevskaia O N, Degtiarev E N, Rykov S V

出版信息

Biofizika. 2008 Mar-Apr;53(2):205-12.

PMID:18543762
Abstract

Irradiation by near-UV light at 77 K of aqueous solutions of inorganic phosphate in weakly acidic conditions in the presence of the photosensitizers adenine and adenosine diphosphate results in the formation of free radicals of these compounds, photosensitized free radicals of phosphate itself, and H* and OH* radicals. The relative concentrations of free radical products were estimated by the analysis of total ESR signals registered in the region of g = 2.00 in the photosystems Ade + Pi and Adphi + Pi using the original computer program of ESR spectra simulation.

摘要

在光敏剂腺嘌呤和二磷酸腺苷存在的情况下,于弱酸性条件下,对无机磷酸盐的水溶液在77 K温度下进行近紫外光照射,会导致这些化合物的自由基、磷酸盐自身的光敏自由基以及H和OH自由基的形成。通过使用电子自旋共振(ESR)光谱模拟的原始计算机程序,分析在光系统Ade + Pi和Adphi + Pi中g = 2.00区域记录的总ESR信号,估算自由基产物的相对浓度。

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