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[培养基氧化还原电位对厌氧细菌生长和代谢的影响]

[Effect of the medium redox potential on the growth and metabolism of anaerobic bacteria].

作者信息

Vasilian A, Trchunian A

出版信息

Biofizika. 2008 Mar-Apr;53(2):281-93.

Abstract

Based on the available literature data on a decrease in the redox potential of medium to low negative values and a decrease in pH during the growth of sugar-fermenting anaerobic bacteria, it was concluded that these processes cannot be described by the theory of redox potential. A theory was developed according to which the regulation of bacterial metabolism is accomplished through changes in the redox potential. The theory considers the redox potential as a factor determining the growth of anaerobic bacteria, which is regulated by oxidizers and reducers. The assumption is put forward that, under anaerobic conditions, bacteria are sensitive to changes in the redox potential and have a redox taxis. The effect of the redox potential on the transport of protons and other substances through membranes and the activity of membrane-bound enzymes, including the proton F1-F0-ATPase, whose mechanisms of action involve changes in the proton conductance of the membrane, the generation of proton-driving force, and dithiol-disulfide transitions in proteins was studied.

摘要

基于关于在发酵糖的厌氧细菌生长过程中培养基氧化还原电位降至中低负值以及pH值下降的现有文献数据,得出这些过程无法用氧化还原电位理论来描述的结论。由此发展出一种理论,即细菌代谢的调节是通过氧化还原电位的变化来实现的。该理论将氧化还原电位视为决定厌氧细菌生长的一个因素,而这一因素由氧化剂和还原剂调节。提出了这样的假设:在厌氧条件下,细菌对氧化还原电位的变化敏感并具有氧化还原趋性。研究了氧化还原电位对质子和其他物质通过膜的转运以及膜结合酶活性的影响,这些膜结合酶包括质子F1 - F0 - ATP酶,其作用机制涉及膜质子传导性的变化、质子驱动力的产生以及蛋白质中二硫键 - 二硫醇的转变。

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