Khater Regina H, Atanassova Pepa K, Anastassov Yourii K
Unit of Plastic and Craniofacial Surgery, St. George University Hospital, Plovdiv, Bulgaria.
Folia Med (Plovdiv). 2008;50(1):29-34.
This study presents the results of a clinicomorphological study of autologous fat transplantation in the face region. The aim was to investigate and compare adipose tissue morphology after it was purified by two methods--centrifugation and serum lavage, and to find a correlation between the histological outcomes and the postoperative results.
The evaluation was performed on a series of 30 patients assigned into two groups of 15 patients. Centrifuged adipose tissue was transplanted in one of the groups, and noncentrifuged (saline solution washed) in the other group. In all cases fat was obtained by syringe liposuction of subgluteal zones, processed by one of the two methods and then transplanted. Part of the material was fixed between vitaline membranes and embedded in paraffin. Histological analysis was performed after hematoxylin eosin staining of the slides. Post-operative effect was assessed by comparison of the pre- and postoperative photos on a 4-grade scale.
The morphological analysis revealed that adipose tissue was damaged by neither of the purification methods but the serum lavage preserved greater number of non-differentiated fat cells and connective tissue fragments. Furthermore, our clinical results suggested clearly that the noncentrifuged adipose tissue transplantation was more advantageous than the other type of transplantation.
The present study is an attempt to make a contribution towards a better understanding of the mechanisms involved in fat graft preservation.
本研究展示了面部自体脂肪移植的临床形态学研究结果。目的是研究和比较经离心和血清冲洗两种方法纯化后的脂肪组织形态，并找出组织学结果与术后效果之间的相关性。
对30例患者进行评估，分为两组，每组15例。一组移植经离心的脂肪组织，另一组移植未离心（用生理盐水冲洗）的脂肪组织。所有病例均通过臀下区域注射器吸脂获取脂肪，采用两种方法之一进行处理后再移植。部分材料固定在维它命膜之间并嵌入石蜡。对玻片进行苏木精-伊红染色后进行组织学分析。通过比较术前和术后照片，以4级量表评估术后效果。
形态学分析表明，两种纯化方法均未损伤脂肪组织，但血清冲洗保留了更多未分化的脂肪细胞和结缔组织碎片。此外，我们的临床结果清楚地表明，未离心脂肪组织移植比另一种移植更具优势。
本研究旨在为更好地理解脂肪移植保存机制做出贡献。
