Debuisson Damien, Treizebré Anthony, Houssin Timothée, Leclerc Eric, Bartès-Biesel Dominique, Legrand Dominique, Mazurier Joel, Arscott Steve, Bocquet Bertrand, Senez Vincent
Institut d'Electronique, de Microélectronique et de Nanotechnologie, IEMN/CNRS-USTL-ISEN, University of Lille, Villeneuve d'Ascq, France.
Physiol Meas. 2008 Jun;29(6):S213-25. doi: 10.1088/0967-3334/29/6/S19. Epub 2008 Jun 10.
Nanoscale probes have been developed for the online characterization of the electrical properties of biological cells by dielectric spectroscopy. Two types of sensors have been designed and fabricated. The first one is devoted to low (<10 MHz) frequency range analysis and consists of gold nanoelectrodes. The second one works for high (>40 Hz) frequency range analysis and consists of a gold nanowire. The patterning of the sensors is performed by electron beam lithography. These devices are integrated in a microfluidic channel network for the manipulation of the cells and for the improvement of the performances of the sensors. These devices are used for the analysis of a well-characterized biological model in the area of the ligand-receptor interaction. The purpose is to monitor the interaction between the lactoferrin (the ligand) and the nucleolin and sulfated proteoglycans (the receptors) present or not on a set of mutant Chinese hamster ovary cell lines and their following internalization into the cytoplasm. Initial measurements have been performed with this microsystem and they demonstrate its capability for label-free, real-time, analysis of a dynamic mechanism involving biological cells.
已开发出纳米级探针，用于通过介电谱对生物细胞的电学性质进行在线表征。设计并制造了两种类型的传感器。第一种用于低（<10 MHz）频率范围分析，由金纳米电极组成。第二种用于高（>40 Hz）频率范围分析，由金纳米线组成。传感器的图案化通过电子束光刻进行。这些装置集成在微流体通道网络中，用于操纵细胞并提高传感器的性能。这些装置用于分析配体 - 受体相互作用领域中一个特征明确的生物模型。目的是监测乳铁蛋白（配体）与一组突变中国仓鼠卵巢细胞系上存在或不存在的核仁素和硫酸化蛋白聚糖（受体）之间的相互作用，以及它们随后内化到细胞质中的情况。已经使用这个微系统进行了初步测量，这些测量证明了其对涉及生物细胞的动态机制进行无标记、实时分析的能力。
