Fernandes Paulo Manuel Pêgo, Said Marcelo Manzano, Pazetti Rogério, Moreira Luis Felipe Pinho, Jatene Fabio Biscegli
FMUSP, University of São Paulo School of Medicine - São Paulo, Brazil.
J Bras Pneumol. 2008 May;34(5):273-9. doi: 10.1590/s1806-37132008000500005.
To evaluate the effects of azathioprine on the mucociliary system in a model of bronchial section and anastomosis in rats.
Thirty-six male Wistar-Furth rats were submitted to left bronchial section and anastomosis and divided into two groups to receive either saline solution or azathioprine. After 7, 15 and 30 days of treatment, six animals from each group were killed, after which in situ mucociliary transport velocity, in vitro mucus transportability, and contact angle of mucus in the right (intact) and left (sectioned) bronchi were measured.
In situ mucociliary transport velocity was significantly lower in the sectioned bronchi than in the intact bronchi (p < 0.001). In situ mucociliary transport velocity was lower in the intact bronchi of the animals treated with azathioprine for 7 days (p < 0.05), and those bronchi presented full recovery after 30 days of treatment. The contact angle was higher in the mucus samples collected from the sectioned bronchi of the animals treated with saline solution for 30 days (p < 0.001), which is in accordance with the decreased in vitro mucus transportability observed in the same animals (p < 0.001).
We conclude that, in the sectioned bronchi of rats, treatment with azathioprine causes only transitory impairment of mucociliary transport, whereas administration of saline solution impairs mucociliary transport for up to 30 days. In addition, azathioprine protects against alterations in mucus surface properties.
在大鼠支气管切断与吻合模型中评估硫唑嘌呤对黏液纤毛系统的影响。
36只雄性Wistar - Furth大鼠接受左支气管切断与吻合术，并分为两组，分别给予生理盐水或硫唑嘌呤。治疗7、15和30天后，每组处死6只动物，然后测量右（完整）支气管和左（切断）支气管的原位黏液纤毛运输速度、体外黏液运输能力以及黏液接触角。
切断支气管的原位黏液纤毛运输速度显著低于完整支气管（p < 0.001）。用硫唑嘌呤治疗7天的动物，其完整支气管的原位黏液纤毛运输速度较低（p < 0.05），但这些支气管在治疗30天后完全恢复。从用生理盐水治疗30天的动物的切断支气管收集的黏液样本中，接触角更高（p < 0.001），这与在相同动物中观察到的体外黏液运输能力下降一致（p < 0.001）。
我们得出结论，在大鼠切断的支气管中，硫唑嘌呤治疗仅导致黏液纤毛运输的短暂损害，而给予生理盐水会损害黏液纤毛运输长达30天。此外，硫唑嘌呤可防止黏液表面性质的改变。
