Department of Chemical and Biochemical Engineering, Faculty of Engineering Science, The University of Western Ontario, London, Ontario, Canada N6A 5B9.
Biotechnol Bioeng. 1982 Apr;24(4):941-53. doi: 10.1002/bit.260240414.
This article examines the potential of Jerusalem artichoke as a source for ethanol and single-cell protein SCP. In addition, experimental results are presented on batch fermentation kinetics employing two strains of Kluyveromyces marxianus and one strain of Saccharomyces rosei grown on the extract derived from the tubers of Jerusalem artichoke. Of the three cultures examined, Kluyveromyces marxianus UCD (FST) 55-82 was found to be the best producer of ethanol grown in a simple medium at 35 degrees C. The ethanol production was found to be growth-associated having a mu(max) = 0.41. h(-1) and the ethanol and biomass yields were determined to be Y(p/s) = 0.45 (88% of the theoretical) and Y(x/s) = 0.04 with 92% of the original sugars utilized. On the basis of carbohydrate yields of Jerusalem artichoke reported in the literature and these batch kinetic studies with K. maxxianus, the calculated ethanol yields were found to range from 1400 kg ethanol acre (-1) yr(-1)to a maximum of 2700 kg ethanol acre (-1) yr(-1). The SCP yields for K. marxianus were calculated to range between 130 to 250 kg dry wt cell acre (-1) yr(-1). The potential for developing an integrated process to produce ethanol and SCP is also discussed.
本文探讨了洋蓟作为乙醇和单细胞蛋白（SCP）来源的潜力。此外，还介绍了利用从洋蓟块茎中提取的提取物培养两种克鲁维酵母（Kluyveromyces marxianus）和一株罗斯酵母（Saccharomyces rosei）的分批发酵动力学实验结果。在所研究的三种培养物中，发现克鲁维酵母 UCD（FST）55-82 在 35°C 的简单培养基中生长时，是乙醇产量最高的菌株。乙醇的生产与生长相关，μ(max)为 0.41 h(-1)，乙醇和生物量的产率分别为 Y(p/s) = 0.45（理论值的 88%）和 Y(x/s) = 0.04，利用了 92%的原始糖。根据文献中报道的洋蓟的碳水化合物产率和这些克鲁维酵母的分批动力学研究，计算出的乙醇产率范围为 1400 至 2700 公斤乙醇/英亩/年。计算出的克鲁维酵母 SCP 产率范围在 130 至 250 公斤干重细胞/英亩/年之间。还讨论了开发生产乙醇和 SCP 的集成工艺的潜力。
