Zhu Min, Zhang Li-Shi, Pei Xiao-Fang, Xu Xin
Huaxi School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China.
Biomed Environ Sci. 2008 Apr;21(2):157-62. doi: 10.1016/S0895-3988(08)60022-7.
To prepare and evaluate novel chlorine dioxide-based disinfectant powder in single-pack that is more convenient for use and transportation.
Orthogonal experiment was performed to determine the recipe of the disinfectant powder. Stability test, suspension quantitative bactericidal test, simulation field trial, and animal toxicity test were carried out to observe its bactericidal and toxicological effects.
The orthogonal experiment showed that the type of water solution had no effect on the disinfectant powder and the best ratio of sodium chlorite to solid acid was 1:3. Ten grams of the disinfectant powder was fully dissolved in 20 mL water for 2 min, and diluted to 500 mL in water. After 5-10 min, the concentration of chlorine dioxide (ClO2) solution was 266 mg/L to 276 mg/L. After stored at 54 degrees C for 14 d, the average concentration of ClO2 was decreased by 5.03%. Suspension quantitative bactericidal test showed that the average killing logarithm (KL) value for both Staphylococcus aureus and Escherichia coli in 100 mg/L ClO2 solution for 2 min was over 5.00. in simulation field trial, the average descending KL value for Escherichia coli in the solution containing 100 mg/L ClO2 for 5 min was over 3.00. The mouse acute LD50 in the solution 5 times exceeded 5000 mg/kg. The disinfectant powder was not toxic and irritative to rabbit skin and had no mutagenic effect on mouse marrow polychromatic erythrocytes (PCE).
The stability and bactericidal efficacy of solid chlorine dioxide-based disinfectant powder in single-pack are good. The solution containing 100 mg/L ClO2 can kill vegetative forms of bacteria. The concentration of ClO2 on the disinfecting surface of objects is 100 mg/L. The disinfectant powder is not toxic and irritative.
制备并评价一种使用和运输更方便的新型单包装二氧化氯基消毒粉。
采用正交试验确定消毒粉配方。进行稳定性试验、悬液定量杀菌试验、模拟现场试验和动物毒性试验，观察其杀菌和毒理学效果。
正交试验表明，水溶液类型对消毒粉无影响，最佳亚氯酸钠与固体酸比例为1:3。将10克消毒粉在20毫升水中完全溶解2分钟，然后用水稀释至500毫升。5 - 10分钟后，二氧化氯（ClO2）溶液浓度为266毫克/升至276毫克/升。在54℃储存14天后，ClO2平均浓度下降5.03%。悬液定量杀菌试验表明，100毫克/升ClO2溶液作用2分钟对金黄色葡萄球菌和大肠杆菌的平均杀灭对数（KL）值均超过5.00。模拟现场试验中，含100毫克/升ClO2溶液作用5分钟对大肠杆菌的平均下降KL值超过3.00。该溶液对小鼠急性经口半数致死量（LD50）5次均超过5000毫克/千克。消毒粉对兔皮肤无毒性和刺激性，对小鼠骨髓嗜多染红细胞（PCE）无致突变作用。
单包装固体二氧化氯基消毒粉稳定性和杀菌效果良好。含100毫克/升ClO2的溶液可杀灭细菌繁殖体。物体消毒表面ClO2浓度为100毫克/升。消毒粉无毒且无刺激性。
