苦味叶下珠的免疫调节作用及其在治疗小鼠抗药性约氏疟原虫（多重耐药）感染中的潜力。
Immunomodulator effect of picroliv and its potential in treatment against resistant Plasmodium yoelii (MDR) infection in mice.
作者信息
Dwivedi Varun, Khan Arif, Vasco Azevedo, Fatima Nishat, Soni Vishal Kumar, Dangi Anil, Misra-Bhattacharya Shailja, Owais Mohammad
机构信息
Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh, India.
出版信息
Pharm Res. 2008 Oct;25(10):2312-9. doi: 10.1007/s11095-008-9631-2. Epub 2008 Jun 13.
PURPOSE
The present study was envisaged to evaluate potential of combination therapy comprising of immunomodulator picroliv and antimalarial chloroquine against drug resistant Plasmodium yoelii (P. yoelii) infection in BALB/c mice.
METHODS
The immunomodulatory potential of picroliv was established by immunizing animals with model antigen along with picroliv. Immune response was assessed using T-cell proliferation assay and also by determining the antibody isotype-profile induced in the immunized mice. In the next set of experiment, prophylactic potential of picroliv to strengthen antimalarial properties of chloroquine against P. yoelii (MDR) infection in BALB/c mice was assessed.
RESULTS
T-cell proliferation as well as antibody production study reveals that picroliv helps in evoking strong immuno-potentiating response against model antigen in the immunized mice. Co-administration of picroliv enhances efficacy of CHQ against experimental murine malaria.
CONCLUSION
The activation of host immune system can increase the efficacy of chloroquine for suppression of drug resistant malaria infection in BALB/c mice.
目的
本研究旨在评估免疫调节剂苦味叶下珠素（picroliv）和抗疟药氯喹联合治疗对BALB/c小鼠耐药物约氏疟原虫（P. yoelii）感染的潜在作用。
方法
通过用模型抗原与苦味叶下珠素一起免疫动物来确定苦味叶下珠素的免疫调节潜力。使用T细胞增殖试验并通过测定免疫小鼠中诱导的抗体同种型谱来评估免疫反应。在接下来的一组实验中，评估了苦味叶下珠素增强氯喹对BALB/c小鼠约氏疟原虫（多重耐药）感染的抗疟特性的预防潜力。
结果
T细胞增殖以及抗体产生研究表明，苦味叶下珠素有助于在免疫小鼠中引发针对模型抗原的强烈免疫增强反应。苦味叶下珠素与氯喹联合使用可提高氯喹对实验性鼠疟的疗效。
结论
宿主免疫系统的激活可提高氯喹在BALB/c小鼠中抑制耐药疟原虫感染的疗效。
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