Neuronal survival and neurite growth in cultured cerebral explants: assessment of the effect of cytosine arabinoside using improved stereology.

Using improved stereological procedures, based on measurements of the total reference volume, the effect of cytosine arabinoside (AraC) on neurons and non-neuronal cells in rat cerebral explants has been reinvestigated. These analyses revealed that, in the AraC-treated explants, neurons support a significantly increased volume of neurites, while the volume of non-neuronal cells in the outgrowth zone is significantly decreased. The total number of neurons is similar in the control and treated explants. Since AraC primarily affects dividing cells, these data suggest that the increased volume of neurites per neuron may be mediated through a curtailment of non-neuronal cell division in the AraC-treated explants. This volumetric approach represents a major improvement on current morphometric approaches for quantifying cultured explants of nervous tissue, and provides important evidence that the previously postulated effects of AraC are real ones.