Oorschot D E
Department of Anatomy, University of Otago Medical School, Dunedin, New Zealand.
Exp Brain Res. 1989;78(1):132-8. doi: 10.1007/BF00230692.
Fetal rat brain neurons are reported to show enhanced neurite development when treated with the mitotic inhibitors fluorodeoxyuridine (FUdR) or cytosine arabinoside (AraC). For AraC-treated rat cerebral explants, increased neurite growth occurs along with a change in the composition of the non-neuronal cell population and diminished non-neuronal cell proliferation. FUdR-treated rat cerebral explants were therefore cultured in an attempt to determine whether FUdR encourages neurite outgrowth by changing the composition and number of non-neuronal cells. Quantitative morphological analyses revealed a significant decrease in the incidence of non-neuronal cells, and an increase in neurite outgrowth for the FUdR-treated explants. These explants also exhibited an increased proportion of protoplasmic astrocytic-epithelial cells and a decreased proportion of fibroblastic-reactive microglial cells. Thus, FUdR may encourage neurite outgrowth through a curtailment of non-neuronal cell proliferation (primarily by fibroblastic-reactive microglial cells) and through the creation of a non-neuronal cell environment consisting almost entirely of protoplasmic astrocytes.
据报道，用有丝分裂抑制剂氟脱氧尿苷（FUdR）或阿糖胞苷（AraC）处理时，胎鼠脑神经元的神经突发育会增强。对于用AraC处理的大鼠脑外植体，神经突生长增加的同时非神经元细胞群体的组成发生变化，且非神经元细胞增殖减少。因此，对用FUdR处理的大鼠脑外植体进行培养，以确定FUdR是否通过改变非神经元细胞的组成和数量来促进神经突生长。定量形态学分析显示，用FUdR处理的外植体中，非神经元细胞的发生率显著降低，神经突生长增加。这些外植体还表现出原浆性星形胶质上皮细胞比例增加，成纤维细胞反应性小胶质细胞比例降低。因此，FUdR可能通过减少非神经元细胞增殖（主要是成纤维细胞反应性小胶质细胞）以及通过创建几乎完全由原浆性星形胶质细胞组成的非神经元细胞环境来促进神经突生长。
