Fox C A, Ross L R, Handa R J, Jacobson C D
Department of Veterinary Anatomy and Molecular, Cellular, Iowa State University, Ames 50011.
Brain Res. 1991 Apr 12;546(1):96-105. doi: 10.1016/0006-8993(91)91163-u.
The Brazilian opossum (Monodelphis domestica) is a small, pouchless marsupial whose young are born in an immature, sexually undifferentiated state. Etgen and Fadem, and Handa and coworkers have biochemically detected and characterized estrogen receptors in the forebrain of the Brazilian opossum. In this study, we have examined the distribution of estrogen receptor-like immunoreactive (ER-LI) cells in the brains of gonadectomized male and female Brazilian opossums using Abbott H222 rat monoclonal estrogen receptor antibody (H222 is a gift of Abbott Labs). An indirect immunohistochemical procedure employing the Vectastain Elite system and a nickel-enhanced DAB chromogen was used. A large number of ER-LI cell nuclei were observed in the medial preoptic area, ventral septal nucleus, medial division of the bed nucleus of the stria terminalis, lateral part of the ventromedial hypothalamus, premammillary nucleus, arcuate nucleus, posterior amygdaloid nucleus, and the midbrain central grey. Lower numbers of ER-LI cell nuclei were observed in the intermediate subdivision of the lateral septal nucleus, and in the anterior, medial, and posterior cortical amygdaloid nuclei. The anatomical distribution of ER-LI in the Brazilian opossum brain is similar to that which has been reported for estrogen binding sites following biochemical analysis. Based on these findings, we believe specific regions of the Brazilian opossum brain may serve as substrata for the action of estrogen in the adult. In addition, these results are supportive of the use of this animal model to investigate the organizational effects of estrogen on the developing central nervous system.
巴西负鼠（Monodelphis domestica）是一种小型无袋有袋动物，其幼崽出生时处于未成熟、性未分化状态。埃特根和法德姆以及汉达及其同事已通过生物化学方法在巴西负鼠的前脑中检测并鉴定了雌激素受体。在本研究中，我们使用雅培H222大鼠单克隆雌激素受体抗体（H222由雅培实验室提供），检测了去势雄性和雌性巴西负鼠大脑中雌激素受体样免疫反应性（ER-LI）细胞的分布。采用了使用Vectorstain Elite系统和镍增强DAB显色剂的间接免疫组织化学方法。在内侧视前区、腹侧隔核、终纹床核内侧部、腹内侧下丘脑外侧部、乳头前核、弓状核、杏仁后核和中脑中央灰质中观察到大量ER-LI细胞核。在外侧隔核中间亚区以及杏仁前皮质核、杏仁内侧皮质核和杏仁后皮质核中观察到的ER-LI细胞核数量较少。巴西负鼠大脑中ER-LI的解剖分布与生化分析后报道的雌激素结合位点分布相似。基于这些发现，我们认为巴西负鼠大脑的特定区域可能是雌激素在成年动物中发挥作用的基础。此外，这些结果支持使用这种动物模型来研究雌激素对发育中的中枢神经系统的组织效应。
