Sugino Y, Kanematsu A, Hayashi Y, Haga H, Yoshimura N, Yoshimura K, Ogawa O
Department of Urology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Neurourol Urodyn. 2008;27(6):548-52. doi: 10.1002/nau.20552.
To evaluate the usefulness of a quantification method using filter paper for analyzing minute voided urine of the mouse.
Voided stain on paper (VSOP) method; the correlation between area of stained spot on a filter paper and amount of applied liquid was calculated. Voiding behavior of the mice was analyzed by placing the animal above the same filter paper and recording voided time and area over 2 hr. The usefulness of the VSOP method was tested in analysis of the voiding behavior of five female 7-week-old ddY mice treated with cyclophosphamide (CPM, 150 mg/kg, intraperitoneally) and five control ones, in comparison with the histology of CPM-induced cystitis. Further, the voided volume of male and female ddY mouse ranging from 2 to 13 weeks was assessed.
There was a linear correlation between liquid volume and stained area on the filter paper (y = 16.472x - 22.411, R(2) = 0.9981). Between control mice and those with histologically proven CPM cystitis, there was a significant difference in voided volume (362.7 +/- 51.9 and 127.8 +/- 100.0 microl, < 0.001) and voiding interval (10.30 +/- 3.10 and 4.47 +/- 1.70 min, < 0.001). Voided volume of ddY mice was quantifiable from as early as 2-week old, increased along with their growth and correlated well with their body weight [(voided volume: microl) = 10.8 x (body weight: g) + 32, R(2) = 0.762].
The VSOP method is a useful tool for evaluating voiding behavior of the mouse, including those with small bladder capacity.
评估使用滤纸对小鼠微量晨尿进行分析的定量方法的实用性。
尿渍滤纸法（VSOP）；计算滤纸上染色斑点面积与所加液体量之间的相关性。将动物放置在同一张滤纸上，记录2小时内的排尿时间和面积，以此分析小鼠的排尿行为。通过比较环磷酰胺（CPM，150mg/kg，腹腔注射）处理的5只7周龄雌性ddY小鼠和5只对照小鼠的排尿行为，并与CPM诱导的膀胱炎组织学结果相比较，来测试VSOP方法的实用性。此外，还评估了2至13周龄雄性和雌性ddY小鼠的排尿量。
滤纸上液体量与染色面积之间存在线性相关性（y = 16.472x - 22.411，R² = 0.9981）。对照小鼠和经组织学证实患有CPM膀胱炎的小鼠之间在排尿量（362.7±51.9和127.8±100.0微升，<0.001）和排尿间隔（10.30±3.10和4.47±1.70分钟，<0.001）方面存在显著差异。ddY小鼠的排尿量早在2周龄时就可定量，随其生长而增加，且与体重相关性良好[（排尿量：微升）= 10.8×（体重：克）+ 32，R² = 0.762]。
VSOP方法是评估小鼠排尿行为的有用工具，包括膀胱容量小的小鼠。
