Greenberg David S, Houweling Arthur R, Kerr Jason N D
Department of Cell Physiology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
Nat Neurosci. 2008 Jul;11(7):749-51. doi: 10.1038/nn.2140. Epub 2008 Jun 15.
It is unclear how the complex spatiotemporal organization of ongoing cortical neuronal activity recorded in anesthetized animals relates to the awake animal. We therefore used two-photon population calcium imaging in awake and subsequently anesthetized rats to follow action potential firing in populations of neurons across brain states, and examined how single neurons contributed to population activity. Firing rates and spike bursting in awake rats were higher, and pair-wise correlations were lower, compared with anesthetized rats. Anesthesia modulated population-wide synchronization and the relationship between firing rate and correlation. Overall, brain activity during wakefulness cannot be inferred using anesthesia.
目前尚不清楚在麻醉动物中记录到的持续皮质神经元活动的复杂时空组织与清醒动物之间的关系。因此，我们在清醒和随后麻醉的大鼠中使用双光子群体钙成像来跟踪不同脑状态下神经元群体的动作电位发放，并研究单个神经元如何对群体活动做出贡献。与麻醉大鼠相比，清醒大鼠的发放率和峰电位爆发更高，成对相关性更低。麻醉调节了全群体同步以及发放率与相关性之间的关系。总体而言，不能用麻醉来推断清醒状态下的脑活动。
