Agarwal P K
Department of Chemical Engineering, The University of Adelaide, G. P.O. Box 498, Adelaide, S.A. 5001, Australia.
Biotechnol Bioeng. 1985 Nov;27(11):1554-63. doi: 10.1002/bit.260271106.
A model is proposed for the denaturation of enzymes based on heterogeneous enzyme subpopulations with nonuniform initial activities and nonzero final activities. The more frequently used simple first-order deactivation model is a special case of the model corresponding to the standard deviation of Gaussian distribution of activation energies approaching zero. Parametric studies made with the model are capable of simulating the reported trends in experimental data on the thermal profile and activity time behavior of native as well as modified enzymes with a minimum number of adjustable parameters. The model predictions are found to be in good agreement with data reported in the literature.
基于具有非均匀初始活性和非零终末活性的异质酶亚群，提出了一种酶变性模型。更常用的简单一级失活模型是该模型的一个特例，对应于活化能高斯分布的标准差趋近于零。用该模型进行的参数研究能够以最少数量的可调参数模拟天然酶和修饰酶的热谱和活性时间行为的实验数据中报道的趋势。发现该模型预测与文献报道的数据高度吻合。
