Xu Xuan, Zhong Li-Li, Jiao Su-Min, Liu San-San, Li Yun, Zhang Bing
Department of Pediatrics, People's Hospital of Hunan Province, Changsha 410005, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2008 Jun;10(3):376-80.
To study the expression of heme oxygenase-1 (HO-1) gene and protein and the effect of budesonide (BUD) on the HO-1 expression in lung tissues in rats with asthma.
Fifty male Sprague-Dawley rats were randomly divided into 5 groups: normal control, asthma model, dexamethasone (DXM)-, hemin (HO-1 challenger)-or BUD-treated asthma. The asthma model was prepared by ovalbumin sensitization and challenge. The rats were sacrificed 24 hrs after the last challenge. The blood COHb content,and the total cell count and the percentage of differential cells in bronchoalveolar lavage fluid (BALF) were measured. The expression of HO-1 protein and mRNA in lung tissues was detected with immunohistochemistry and RT-PCR, respectively. The airway inflammation situations were evaluated by histopathology.
The airway inflammatory cell infiltration in the DXM-, hemin- and BUD-treated asthma groups was remarkably alleviated compared with that in the asthma model group. Compared with the normal control group, the expression of HO-1 mRNA and protein in lung tissues and the blood COHb content in the asthma model and the DXM-, hemin- and BUD-treated asthma groups were significantly up-regulated. The DXM-, hemin- and BUD-treated asthma groups showed significantly increased expression of HO-1 protein and mRNA in lung tissues compared with the asthma model group. The blood COHb content in the DXM-and the hemin-treated asthma groups was significantly higher than that in the asthma model group.
The expression of HO-1 protein and mRNA in lung tissues and blood HO-1 activity increased in rats with asthma,suggesting that HO-1 may be involved in the pathogenesis of asthma. HO-1 may have a protective effect against the airway inflammation in asthmatic rats. BUD and DXM can up-regulate the expression of HO-1 protein and mRNA, thus providing protective effects against the airway inflammation in asthmatic rats.
研究血红素加氧酶-1（HO-1）基因和蛋白的表达以及布地奈德（BUD）对哮喘大鼠肺组织中HO-1表达的影响。
50只雄性Sprague-Dawley大鼠随机分为5组：正常对照组、哮喘模型组、地塞米松（DXM）治疗哮喘组、氯化血红素（HO-1激发剂）治疗哮喘组或BUD治疗哮喘组。通过卵清蛋白致敏和激发制备哮喘模型。末次激发后24小时处死大鼠。检测血液中碳氧血红蛋白（COHb）含量、支气管肺泡灌洗液（BALF）中的细胞总数及分类细胞百分比。分别采用免疫组织化学和逆转录-聚合酶链反应（RT-PCR）检测肺组织中HO-1蛋白和mRNA的表达。通过组织病理学评估气道炎症情况。
与哮喘模型组相比，DXM治疗哮喘组、氯化血红素治疗哮喘组和BUD治疗哮喘组的气道炎性细胞浸润明显减轻。与正常对照组相比，哮喘模型组、DXM治疗哮喘组、氯化血红素治疗哮喘组和BUD治疗哮喘组肺组织中HO-1 mRNA和蛋白的表达以及血液中COHb含量均显著上调。与哮喘模型组相比，DXM治疗哮喘组、氯化血红素治疗哮喘组和BUD治疗哮喘组肺组织中HO-1蛋白和mRNA的表达显著增加。DXM治疗哮喘组和氯化血红素治疗哮喘组血液中COHb含量显著高于哮喘模型组。
哮喘大鼠肺组织中HO-1蛋白和mRNA的表达及血液中HO-1活性增加，提示HO-1可能参与哮喘的发病机制。HO-1可能对哮喘大鼠的气道炎症具有保护作用。BUD和DXM可上调HO-1蛋白和mRNA的表达，从而对哮喘大鼠的气道炎症起到保护作用。
