Hendgen-Cotta Ulrike, Grau Marijke, Rassaf Tienush, Gharini Putrika, Kelm Malte, Kleinbongard Petra
Department of Medicine, Division of Cardiology, Pulmology and Vascular Medicine, CardioBioTech Research Group, University Hospital Aachen, Aachen, Germany.
Methods Enzymol. 2008;441:295-315. doi: 10.1016/S0076-6879(08)01216-0.
There is growing evidence for nitric oxide (NO.) being involved in cell signaling and pathology. Much effort has been made to elucidate and characterize the different biochemical reaction pathways of NO.in vivo. However, a major obstacle in assessing the significance of nitrosated species and oxidized metabolites often remains: a reliable analytical technique for the detection of NO. in complex biological matrices. This chapter presents refined methodologies, such as chemiluminescence detection and flow injection analysis, compared with adequate sample processing procedures to reliably quantify and assess the circulating and resident NO(.) pool, consisting of nitrite, nitrate, nitroso, and nitrosylated species.
越来越多的证据表明，一氧化氮（NO.）参与细胞信号传导和病理学过程。人们已付出诸多努力来阐明和表征体内NO.的不同生化反应途径。然而，在评估亚硝化物种和氧化代谢产物的重要性时，一个主要障碍仍然存在：一种用于检测复杂生物基质中NO.的可靠分析技术。本章介绍了诸如化学发光检测和流动注射分析等精细方法，并与适当的样品处理程序进行了比较，以可靠地定量和评估由亚硝酸盐、硝酸盐、亚硝基和亚硝基化物种组成的循环和驻留NO(.)库。
