MacArthur Peter H, Shiva Sruti, Gladwin Mark T
Vascular Medicine Branch, National Heart Lung Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 15;851(1-2):93-105. doi: 10.1016/j.jchromb.2006.12.012. Epub 2007 Jan 5.
Considerable disparities in the reported levels of basal human nitrite and S-nitrosothiols (RSNO) in blood have brought methods of quantifying these nitric oxide (NO) metabolites to the forefront of NO biology. Ozone-based chemiluminescence is commonly used and is a robust method for measuring these species when combined with proper reductive chemistry. The goal of this article is to review existing methodologies for the measurement of nitrite and RSNO by reductive chemiluminescence. Specifically, we discuss in detail the measurement of nitrite and RSNO in biological matrices using tri-iodide and copper(I)/cysteine-based reduction methods coupled to chemiluminescence. The underlying reaction mechanisms, as well as the potential pitfalls of each method are discussed.
血液中所报道的基础人类亚硝酸盐和S-亚硝基硫醇（RSNO）水平存在相当大的差异，这使得这些一氧化氮（NO）代谢物的定量方法成为NO生物学的前沿领域。基于臭氧的化学发光法是常用方法，当与适当的还原化学相结合时，它是测量这些物质的可靠方法。本文的目的是综述通过还原化学发光法测量亚硝酸盐和RSNO的现有方法。具体而言，我们详细讨论了使用三碘化物和基于铜（I）/半胱氨酸的还原方法与化学发光相结合来测量生物基质中的亚硝酸盐和RSNO。讨论了每种方法的潜在陷阱及其潜在的反应机制。
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