Saade Fadi, Buronfosse Thierry, Pradat Pierre, Abdul Fabien, Cova Lucyna
INSERM, Unit 871, 151 Cours Albert Thomas, Université Lyon 1, 69008 Lyon, France.
Vaccine. 2008 Sep 19;26(40):5159-64. doi: 10.1016/j.vaccine.2008.03.086. Epub 2008 Apr 21.
We explored in the duck hepatitis B virus (DHBV) model the impact of duck interferon gamma (Du-IFNgamma) or interleukin 2 (Du-IL2) co-delivery on humoral neutralizing response induced by DNA-based vaccine encoding DHBV preS/S large envelope protein. Co-delivery of either Du-IL2 or Du-IFNgamma encoding plasmids considerably increased the magnitude of anti-preS humoral response. Moreover, co-administration of cytokine genes led to a significant (p<0.001) enhancement of neutralizing anti-DHBV antibody response, which was more pronounced for Du-IFNgamma. Our data suggest that co-delivery of cytokine and envelope protein encoding plasmids will be a valuable approach for the development of a potent therapeutic DNA vaccine against chronic hepatitis B.
我们在鸭乙型肝炎病毒（DHBV）模型中探究了鸭γ干扰素（Du-IFNγ）或白细胞介素2（Du-IL2）共同递送对编码DHBV preS/S大包膜蛋白的DNA疫苗诱导的体液中和反应的影响。Du-IL2或编码Du-IFNγ的质粒的共同递送显著提高了抗preS体液反应的强度。此外，细胞因子基因的共同给药导致中和抗DHBV抗体反应显著增强（p<0.001），这在Du-IFNγ中更为明显。我们的数据表明，细胞因子和包膜蛋白编码质粒的共同递送将是开发一种有效的抗慢性乙型肝炎治疗性DNA疫苗的有价值方法。
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