枯草芽孢杆菌、粪产碱菌和肠杆菌属在液体培养中生产生物乳化剂。
Production of bioemulsifier by Bacillus subtilis, Alcaligenes faecalis and Enterobacter species in liquid culture.
作者信息
Toledo F L, Gonzalez-Lopez J, Calvo C
机构信息
Group of Environmental Microbiology, Department of Microbiology, Institute of Water Research, University of Granada, Spain.
出版信息
Bioresour Technol. 2008 Nov;99(17):8470-5. doi: 10.1016/j.biortech.2007.08.055. Epub 2008 Jun 12.
Three bacterial strains isolated from waste crude oil were selected due to their capacity of growing in the presence of hydrocarbons and production of bioemulsifier. The genetic identification (PCR of the 16S rDNA gene using fD1 and rD1 primers) of these strains showed their affiliation to Bacillus subtilis, Alcaligenes faecalis and Enterobacter sp. These strains were able to emulsify n-octane, toluene, xylene, mineral oils and crude oil, look promising for bioremediation application. Finally, chemical composition, emulsifying activity and surfactant activity of the biopolymers produced by the selected strains were studies under different culture conditions. Our results showed that chemical and functional properties of the bioemulsifiers were affected by the carbon source added to the growth media.
从废原油中分离出的三株细菌菌株因其在碳氢化合物存在下的生长能力和生物乳化剂的产生而被选中。这些菌株的基因鉴定（使用fD1和rD1引物对16S rDNA基因进行PCR）表明它们隶属于枯草芽孢杆菌、粪产碱菌和肠杆菌属。这些菌株能够乳化正辛烷、甲苯、二甲苯、矿物油和原油，在生物修复应用方面看起来很有前景。最后，在不同培养条件下研究了所选菌株产生的生物聚合物的化学成分、乳化活性和表面活性剂活性。我们的结果表明，添加到生长培养基中的碳源会影响生物乳化剂的化学和功能特性。
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