Ghojavand Hossein, Vahabzadeh Farzaneh, Roayaei Emad, Shahraki Alireza Khodabandeh
Department of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran; IOR Research Institute, National Iranian Oil Company, Tehran, Iran.
J Colloid Interface Sci. 2008 Aug;324(1-2):172-6. doi: 10.1016/j.jcis.2008.05.001. Epub 2008 May 10.
The production and properties of a biosurfactant, synthesized by a member of the Bacillus subtilis group (PTCC 1696) which was isolated from an Iranian oil field, have been investigated. The biosurfactant, which was produced as a primary metabolite associated with the growth of PTCC 1696, was able to reduce the surface and interfacial tension of media to 26.7 and 0.1 mN/m, respectively. Crude biosurfactant and acid precipitated biosurfactant have critical micelle concentrations of 10 and 100 mug/ml, respectively. The stability of the biosurfactant at different salinities, pH and temperature and also its emulsifying activity have been investigated. It is an effective surfactant at very low concentrations over a wide range of temperatures, pHs and salt concentrations and also has the ability to emulsify oil, which is essential for enhanced oil recovery.
对从伊朗油田分离出的枯草芽孢杆菌属（PTCC 1696）成员合成的一种生物表面活性剂的生产及特性进行了研究。该生物表面活性剂作为与PTCC 1696生长相关的初级代谢产物产生，能够分别将培养基的表面张力和界面张力降低至26.7和0.1 mN/m。粗制生物表面活性剂和酸沉淀生物表面活性剂的临界胶束浓度分别为10和100 μg/ml。研究了该生物表面活性剂在不同盐度、pH值和温度下的稳定性及其乳化活性。它在很宽的温度、pH值和盐浓度范围内，在极低浓度下就是一种有效的表面活性剂，并且还具有乳化油的能力，这对于提高采油率至关重要。
