Sheffield J B, Graff D
Department of Biology, Temple University, Philadelphia, PA 19122.
Exp Eye Res. 1991 Jun;52(6):733-41. doi: 10.1016/0014-4835(91)90025-a.
During retinal histogenesis, cells and their extensions migrate within the tissue to final positions. In order for the cells to move through the matrix of tissue, space must be made available. We report evidence that extracellular proteolytic activity might be associated with this process. (1) When embryonic chick neural retinal cells are seeded onto a substrate of rhodamine conjugated fluorescent gelatin, the tips of growing neurites remove the fluorescence from the substrate. (2) Latent gelatinolytic activity can be identified with soluble assays of homogenates of embryonic chick neural retina. (3) Zymogram analysis demonstrates the presence of high molecular weight bands of proteolytic activity. The activity is inhibited by 1.10 phenanthroline, suggesting that it is due to a metalloproteinase. Activity can be detected in supernatants of retinal cells grown in vitro. Gelatinolysis is not the only proteolytic activity detected in the retina. Addition of plasminogen to zymograms results in an additional band of activity.
在视网膜组织发生过程中，细胞及其突起在组织内迁移至最终位置。为使细胞能够在组织基质中移动，必须提供空间。我们报告了细胞外蛋白水解活性可能与该过程相关的证据。（1）当将胚胎鸡神经视网膜细胞接种到罗丹明偶联的荧光明胶底物上时，生长中的神经突尖端会去除底物上的荧光。（2）潜在的明胶溶解活性可以通过胚胎鸡神经视网膜匀浆的可溶性测定来鉴定。（3）酶谱分析表明存在蛋白水解活性的高分子量条带。该活性被1,10 - 菲咯啉抑制，表明它是由金属蛋白酶引起的。在体外培养的视网膜细胞的上清液中可以检测到活性。明胶溶解并不是在视网膜中检测到的唯一蛋白水解活性。向酶谱中添加纤溶酶原会产生一条额外的活性条带。
