Ciofani Gianni, Migliore Antonio, Raffa Vittoria, Menciassi Arianna, Dario Paolo
CRIM Lab, Center for Research In Microengineering, Scuola Superiore Sant'Anna, Viale Rinaldo Piaggio, Pontedera, Italy.
J Biosci Bioeng. 2008 May;105(5):536-44. doi: 10.1263/jbb.105.536.
Low-shear perfusion and compartmental systems are very important conceptual designs for the realization of high-density cell cultures and cocultures, thus allowing very realistic models and therefore reliable results. In this work, we combines low-shear perfusion and multi-compartments in one device. We also describe the design, fabrication and validation of a bicompartmental, low-perfusion culture chamber specifically designed for applications in various fields, such as tissue engineering, pharmacology, molecular biology, pharmacokinetics, nanomedicine, and drug delivery testing. The device properties in terms of flow features and metabolite exchange capabilities are fully characterized. In vitro tests performed on cell lines confirm the effectiveness of the device as an experimental tool for measurements in dynamic and multiple-cell cultures and cocultures.
低剪切力灌注和隔室系统是实现高密度细胞培养和共培养的非常重要的概念性设计，从而能够构建非常逼真的模型并获得可靠的结果。在这项工作中，我们将低剪切力灌注和多隔室整合在一个装置中。我们还描述了一种双隔室、低灌注培养室的设计、制造和验证，该培养室专门设计用于组织工程、药理学、分子生物学、药代动力学、纳米医学和药物递送测试等各个领域的应用。对该装置的流动特性和代谢物交换能力等装置特性进行了全面表征。对细胞系进行的体外测试证实了该装置作为动态和多细胞培养及共培养测量实验工具的有效性。
