Identification of Enterococcus species by melting curve analysis of restriction fragments.
作者信息
Martín Belén, Garriga Margarita, Aymerich Teresa
机构信息
Institute for Food and Agricultural Research and Technology (IRTA), Finca Camps i Armet, E-17121 Monells, Spain.
出版信息
J Microbiol Methods. 2008 Sep;75(1):145-7. doi: 10.1016/j.mimet.2008.05.005. Epub 2008 May 13.
PMID:18558446
Abstract
A new method for the identification of Enterococcus species has been developed. It combines PCR amplification of sodA gene and 16S-23S intergenic spacer region with restriction enzyme digestion followed by a melting curve analysis of the restriction fragments (MCARF). All strains analyzed were correctly identified by MCARF. This method was proved to be a reliable enterococcal identification tool.
摘要
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