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当代修饰小鼠基因组的方法。

Contemporary approaches for modifying the mouse genome.

作者信息

Adams David J, van der Weyden Louise

机构信息

Experimental Cancer Genetics, The Wellcome Trust Sanger Institute, Hinxton, United Kingdom.

出版信息

Physiol Genomics. 2008 Aug 15;34(3):225-38. doi: 10.1152/physiolgenomics.90242.2008. Epub 2008 Jun 17.

DOI:10.1152/physiolgenomics.90242.2008
PMID:18559964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2519963/
Abstract

The mouse is a premiere experimental organism that has contributed significantly to our understanding of vertebrate biology. Manipulation of the mouse genome via embryonic stem (ES) cell technology makes it possible to engineer an almost limitless repertoire of mutations to model human disease and assess gene function. In this review we outline recent advances in mouse experimental genetics and provide a "how-to" guide for those people wishing to access this technology. We also discuss new technologies, such as transposon-mediated mutagenesis, and resources of targeting vectors and ES cells, which are likely to dramatically accelerate the pace with which we can assess gene function in vivo, and the progress of forward and reverse genetic screens in mice.

摘要

小鼠是一种重要的实验生物，对我们理解脊椎动物生物学有重大贡献。通过胚胎干细胞（ES）技术对小鼠基因组进行操作，使得构建几乎无限多样的突变体成为可能，从而用于模拟人类疾病和评估基因功能。在本综述中，我们概述了小鼠实验遗传学的最新进展，并为希望使用这项技术的人员提供一份“操作指南”。我们还讨论了诸如转座子介导的诱变等新技术，以及靶向载体和ES细胞资源，这些很可能极大地加快我们在体内评估基因功能的速度，以及小鼠正向和反向遗传筛选的进程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/24c6e99ea21c/zh70110832510006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/e38d966ecfd9/zh70110832510001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/3424beb84cd1/zh70110832510002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/385dde15e050/zh70110832510003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/2f26805fa7e1/zh70110832510004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/2cdf398b14e1/zh70110832510005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/24c6e99ea21c/zh70110832510006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/e38d966ecfd9/zh70110832510001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/3424beb84cd1/zh70110832510002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/385dde15e050/zh70110832510003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/2f26805fa7e1/zh70110832510004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/2cdf398b14e1/zh70110832510005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2128/2519963/24c6e99ea21c/zh70110832510006.jpg

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