Erzinger Gilmar Sidney, Vitolo Michele
Pharmacy School, University of Joinville, Santa Catarina, SC, Brazil.
Appl Biochem Biotechnol. 2006 Mar;131(1-3):787-94. doi: 10.1385/ABAB:131:1:787.
The conversion of glucose and fructose into gluconic acid (GA) and sorbitol (SOR) was conducted in a batch reactor with free (CTAB-treated or not) or immobilized cells of Zymomonas mobilis. High yields (more than 90%) of gluconic acid and sorbitol were attained at initial substrate concentration of 600 g/L (glucose plus fructose at 1:1 ratio), using cells with glucose-fructose-oxidoreductase activity of 75 U/L. The concentration of the products varied hyperbolically with time according to the equations (GA)=t(GA)(max)/(W(GA) +t), (SOR)=t (SOR)(max)/(W(Sor)+t), v(GA)=[W(GA) (GA)(max)]/(W(GA)+t)(2) and V(SOR)=[W(SOR) (SOR)(max)]/(W(SOR)+t)(2). Taking the test carried out with free CTAB-treated cells as an example, the constant parameters were (GA)(max)= 541 g/L, (SOR)(max)=552 g/L, W(GA)=4.8h, W(SOR)=4.9h, upsilon(GA)=112.7 g/L. and upsilon(SOR)=112.7 g/L.
葡萄糖和果糖转化为葡萄糖酸（GA）和山梨醇（SOR）的反应在间歇式反应器中进行，使用的是游离（经或未经十六烷基三甲基溴化铵（CTAB）处理）或固定化的运动发酵单胞菌细胞。在初始底物浓度为600 g/L（葡萄糖和果糖按1:1比例）时，使用葡萄糖-果糖氧化还原酶活性为75 U/L的细胞，可获得高产率（超过90%）的葡萄糖酸和山梨醇。根据方程(GA)=t(GA)(max)/(W(GA) +t)、(SOR)=t (SOR)(max)/(W(Sor)+t)、v(GA)=[W(GA) (GA)(max)]/(W(GA)+t)(2)和V(SOR)=[W(SOR) (SOR)(max)]/(W(SOR)+t)(2)，产物浓度随时间呈双曲线变化。以对游离CTAB处理细胞进行的测试为例，常数参数为(GA)(max)= 541 g/L、(SOR)(max)=552 g/L、W(GA)=4.8h、W(SOR)=4.9h、υ(GA)=112.7 g/L和υ(SOR)=112.7 g/L。
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