Tůma Petr, Samcová Eva, Duska Frantisek
Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
J Sep Sci. 2008 Jul;31(12):2260-4. doi: 10.1002/jssc.200700655.
CE with capacitively coupled contactless conductivity detection (C(4)D) was used to determine waste products of the nitrogen metabolism (ammonia and creatinine) and of biogenic inorganic cations in samples of human urine. The CE separation was performed in two BGEs, consisting of 2 M acetic acid + 1.5 mM crown ether 18-crown-6 (BGE I) and 2 M acetic acid + 2% w/v PEG (BGE II). Only BGE II permitted complete separation of all the analytes in a model sample and in real urine samples. The LOD values for the optimized procedure ranged from 0.8 microM for Ca(2+) and Mg(2+) to 2.9 microM for NH(4)(+) (in terms of mass concentration units, from 7 microg/L for Li(+) to 102 microg/L for creatinine). These values are adequate for determination of NH(4)(+), creatinine, Na(+), K(+), Ca(2+) and Mg(2+) in real urine samples.
采用带有电容耦合非接触式电导检测（C(4)D）的毛细管电泳法测定人尿样中氮代谢废物（氨和肌酐）以及生物源无机阳离子。毛细管电泳分离在两种背景电解质（BGE）中进行，分别为2 M乙酸 + 1.5 mM冠醚18 - 冠 - 6（BGE I）和2 M乙酸 + 2% w/v聚乙二醇（BGE II）。只有BGE II能够在模型样品和实际尿样中实现所有分析物的完全分离。优化后的方法的检测限（LOD）值范围为：Ca(2+)和Mg(2+)为0.8 microM，NH(4)(+)为2.9 microM（以质量浓度单位计，Li(+)为7 microg/L，肌酐为102 microg/L）。这些值足以用于实际尿样中NH(4)(+)、肌酐、Na(+)、K(+)、Ca(2+)和Mg(2+)的测定。
