Léger J, Chevalier J, Larue C, Gautier P, Planchenault J, Aumaître E, Messner P, Puech P, Saccavini J C, Pau B
INSERM, Faculté de Pharmacie, Montpellier, France.
J Am Coll Cardiol. 1991 Aug;18(2):473-84. doi: 10.1016/0735-1097(91)90603-7.
The use of three different monoclonal antibodies specific for human ventricular myosin heavy chains in the visualization of the location and extent of necrosis in dogs with experimental acute myocardial infarction and in humans is described. Using a classic immunohistochemical method or ex vivo analysis of heart slices in dogs with acute myocardial infarction subjected to intravenous injection of unlabeled antimyosin antibodies or antimyosin antibodies labeled with indium-111, it was observed that all antibody fragments specifically reached the targeted necrotic zone less than 2 h after antibody injection and remained bound for up to 24 h. In a limited but significant number of cases (5 of the 12 humans and 11 of 43 dogs), it was possible to image the necrotic zone in vivo as early as 2 to 4 h after antibody injection. In other cases, individual blood clearance variations retarded or even prevented in vivo necrosis detection. Higher antimyosin fixation values were obtained in the necrotic zones in dogs with a rapid blood clearance relative to that of the other dogs. It is concluded that antimyosin antibodies always reached necrotic areas within 2 h. If blood clearance was rapid, in vivo imaging of the necrotic area was possible 2 to 6 h after necrosis, even in humans. In some cases, however, uncontrolled individual variations in the timing required for sufficient blood clearance hampered this rapid in vivo detection of myocardial necrosis.
本文描述了使用三种针对人心室肌球蛋白重链的不同单克隆抗体，来观察实验性急性心肌梗死犬及人类坏死的位置和范围。通过经典免疫组化方法，或对急性心肌梗死犬进行静脉注射未标记抗肌球蛋白抗体或铟 -111 标记的抗肌球蛋白抗体后对心脏切片进行离体分析，观察到所有抗体片段在注射抗体后不到 2 小时即可特异性到达目标坏死区，并持续结合长达 24 小时。在有限但数量可观的病例中（12 名人类患者中的 5 例和 43 只犬中的 11 例），在注射抗体后 2 至 4 小时即可在体内对坏死区进行成像。在其他病例中，个体血液清除率的差异延迟甚至阻碍了体内坏死检测。相对于其他犬，血液清除较快的犬坏死区获得了更高的抗肌球蛋白固定值。结论是抗肌球蛋白抗体总是在 2 小时内到达坏死区域。如果血液清除迅速，即使在人类中，坏死发生后 2 至 6 小时也可在体内对坏死区进行成像。然而，在某些情况下，血液充分清除所需时间的个体差异不受控制，阻碍了心肌坏死的这种快速体内检测。
