Gaudart Narintorn, Ekpo Pattama, Pattanapanyasat Kovit, van Kooyk Yvette, Engering Anneke
Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FEMS Immunol Med Microbiol. 2008 Aug;53(3):359-67. doi: 10.1111/j.1574-695X.2008.00437.x. Epub 2008 Jun 28.
Leptospirosis is a global zoonotic disease, caused by pathogenic Leptospira species including Leptospira interrogans, that causes public health and livestock problems. Pathogenesis, immune response and cellular receptors for Leptospira are not well understood. Interaction of dendritic cells (DCs) with L. interrogans serovar Autumnalis L-643 and BL-6 isolated from leptospirosis patients, and both virulent and avirulent serovar Pyrogenes 2317 strains isolated from animal were investigated. Carbohydrate analysis using lectins showed that all of these leptospires contained high mannose components as a common backbone and DC-SIGN was involved in leptospires' attachment. Interaction of the L. interrogans strains with DCs induced maturation, but had different effects on IL-10, IL-12p70 and tumor necrosis factor (TNF)-alpha production. Both virulent and avirulent Pyrogenes 2317 and Autumnalis BL-6 but not L-643 strains induced IL-12p70 and TNF-alpha production, but minimal IL-10 secretion. These data demonstrated that L. interrogans binds DC-SIGN and induces DCs maturation and cytokine production, which should provide new insights into cellular immune processes during leptospirosis.
钩端螺旋体病是一种全球性人畜共患病，由包括问号钩端螺旋体在内的致病性钩端螺旋体引起，会导致公共卫生和家畜问题。钩端螺旋体的发病机制、免疫反应和细胞受体尚不清楚。研究了树突状细胞（DCs）与从钩端螺旋体病患者分离出的秋季型问号钩端螺旋体L-643和BL-6，以及从动物分离出的强毒和无毒热原性2317菌株之间的相互作用。使用凝集素进行的碳水化合物分析表明，所有这些钩端螺旋体都含有高甘露糖成分作为共同骨架，并且DC-SIGN参与了钩端螺旋体的附着。问号钩端螺旋体菌株与DCs的相互作用诱导了DCs成熟，但对IL-10、IL-12p70和肿瘤坏死因子（TNF）-α的产生有不同影响。强毒和无毒的热原性2317以及秋季型BL-6菌株而非L-643菌株诱导了IL-12p70和TNF-α的产生，但IL-10分泌极少。这些数据表明，问号钩端螺旋体与DC-SIGN结合并诱导DCs成熟和细胞因子产生，这应为钩端螺旋体病期间的细胞免疫过程提供新的见解。
