Na-ngam Narisorn, Kalambaheti Thareerat, Ekpo Pattama, Pitaksajjakul Pannamthip, Jamornthanyawat Natsuda, Chantratita Narisara, Sirisinha Stitaya, Thamlikitkul Visanu, Chaicumpa Wanpen, Yamabhai Montarop, Ramasoota Pongrama
Department of Veterinary Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand.
Southeast Asian J Trop Med Public Health. 2008 May;39(3):443-51.
Random peptide libraries displayed by bacteriophage T7 and M13 were employed to identify mimotopes from 4 monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei. Insert DNA sequences of bound phages selected from four rounds of panning with each MAb revealed peptide sequences corresponding to B. pseudomallei K96243 hypothetical protein BPSL2046, hypothetical protein BpseP_02000035, B. pseudomallei K96243 hypothetical protein BPSS0784, B. pseudomallei 1710b hypothetical protein BURPS1710b_1104, and B. cenocepacia H12424 TonB-dependent siderophore receptor, all located at the outer membrane. The immune responses from all selected phagotopes were significantly higher than that of lipopolysaccharide. The study demonstrates the feasibility of identifying mimotopes through screening of phage-displayed random peptide libraries with B. pseudomallei MAbs.
利用噬菌体T7和M13展示的随机肽库，从4种针对伯克霍尔德菌的单克隆抗体（MAb）中鉴定模拟表位。从四轮与每种单克隆抗体的淘选中选出的结合噬菌体的插入DNA序列，揭示了与伯克霍尔德菌K96243假定蛋白BPSL2046、假定蛋白BpseP_02000035、伯克霍尔德菌K96243假定蛋白BPSS0784、伯克霍尔德菌1710b假定蛋白BURPS1710b_1104以及洋葱伯克霍尔德菌H12424的TonB依赖性铁载体受体相对应的肽序列，这些蛋白均位于外膜。所有选定噬菌体表位的免疫反应均显著高于脂多糖。该研究证明了通过用伯克霍尔德菌单克隆抗体筛选噬菌体展示的随机肽库来鉴定模拟表位的可行性。
