Sandoval-Gío Juan José, Rodríguez-Canul Rossanna, Vidal-Martínez Víctor Manuel
Laboratorio de Inmunología y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN) Unidad Mérida, Carretera Antigua a Progreso Km, 6 AP 73 Cordemex, 97310 Mérida, Yucatán, México.
J Parasitol. 2008 Apr;94(2):404-9. doi: 10.1645/GE-1382.1.
The humoral immune response of the tilapia Oreochromis niloticus was evaluated using a direct ELISA. Serum was tested from fish infected with Cichlidogyrus spp. (Monogenea) and from fish injected intraperitoneally with the Cichlidogyrus spp. antigenic extract, i.e., 150 microl of the Cichlidogyrus spp. saline extract diluted in Freund's complete adjuvant (FCA) (1:1) were inoculated intraperitoneally at day 0, followed by 2 dosages of 50 microl of the same Cichlidogyrus spp. saline extract diluted in Freund's incomplete adjuvant (FIA) (1:1) at weeks 2 and 4, respectively. The humoral response was also evaluated by the double immunodiffusion test (DID) and by serum protein and total immunoglobulin (Ig) determinations. The IgM OD values in the hyperimmune fish were significantly higher than in the infected and uninfected fish groups. In the DID test, a precipitation (antigen-antibody) band was observed between the Cichlidogyrus spp. saline extract and hyperimmune sera, but not with the other groups. Increases in serum protein concentration and total Igs were observed in the immunized fish at weeks 2 and 10 postinjection. Results from this study suggest that tilapia is capable of producing an induced humoral immune response against an antigenic extract of Cichlidogyrus spp.
使用直接酶联免疫吸附测定法（ELISA）评估尼罗罗非鱼（Oreochromis niloticus）的体液免疫反应。检测感染了三代虫属（Cichlidogyrus spp.）（单殖吸虫纲）的鱼以及经腹腔注射三代虫属抗原提取物的鱼的血清。即在第0天腹腔接种150微升在弗氏完全佐剂（FCA）（1:1）中稀释的三代虫属盐水提取物，随后分别在第2周和第4周腹腔注射2剂50微升在弗氏不完全佐剂（FIA）（1:1）中稀释的相同三代虫属盐水提取物。还通过双向免疫扩散试验（DID）以及血清蛋白和总免疫球蛋白（Ig）测定来评估体液反应。超免疫鱼中的IgM光密度值显著高于感染组和未感染组的鱼。在DID试验中，在三代虫属盐水提取物和超免疫血清之间观察到一条沉淀（抗原 - 抗体）带，但其他组未观察到。在注射后第2周和第10周，免疫鱼的血清蛋白浓度和总免疫球蛋白增加。本研究结果表明，罗非鱼能够针对三代虫属的抗原提取物产生诱导性体液免疫反应。
