就诊于不孕不育诊所男性精液中1-7型人类疱疹病毒的流行情况及其与精液参数的相关性。

Prevalence of human herpes virus types 1-7 in the semen of men attending an infertility clinic and correlation with semen parameters.

作者信息

Neofytou Eirini, Sourvinos George, Asmarianaki Maria, Spandidos Demetrios A, Makrigiannakis Antonios

机构信息

Laboratory of Human Reproduction, IVF Clinic, Department of Obstetrics and Gynecology, Faculty of Medicine, University of Crete, Crete, Greece.

出版信息

Fertil Steril. 2009 Jun;91(6):2487-94. doi: 10.1016/j.fertnstert.2008.03.074. Epub 2008 Jun 18.

DOI:10.1016/j.fertnstert.2008.03.074

PMID:18565516

Abstract

OBJECTIVE

To determine the prevalence of herpes viruses in the semen of an asymptomatic male cohort with and without infertility problems and its association with altered semen parameters.

DESIGN

A prospective randomized study.

SETTING

Medical school and IVF clinic.

PATIENT(S): One hundred seventy-two male patients undergoing routine semen analysis: 80 with normal semen parameters (control group) and 92 with abnormal semen parameters.

INTERVENTION(S): Semen samples were collected by masturbation.

MAIN OUTCOME MEASURE(S): The DNA from the Herpesviridae family (herpes simplex virus 1 [HSV-1], herpes simplex virus 2 [HSV-2], Varicella zoster virus [VZV], Epstein-Barr virus [EBV], cytomegalovirus [CMV], human herpes virus type 6 [HHV-6], human herpes virus type 7 [HHV-7]) and routine semen parameters.

RESULT(S): Viral DNA was detected in 143/172 (83.1%) of the total samples for at least one herpes virus: HSV-1, 2.5%; VZV, 1.2%; EBV, 45%; CMV, 62.5%; HHV-6, 70%; HHV-7, 0% in the normal semen samples and HSV-1, 2.1%; VZV, 3.2%; EBV, 39.1%; CMV, 56.5%; HHV-6, 66.3%; HHV-7, 0% in the abnormal semen samples. No association was found between the presence of viral DNA and semen parameters. Interestingly, a statistical significance between leukocytospermia and the presence of EBV DNA was observed.

CONCLUSION(S): The DNA of herpes viruses is frequently detected in the semen of asymptomatic fertile and infertile male patients. Further studies are required to investigate the role of herpes viruses in male factor infertility.

摘要

目的

确定有无不育问题的无症状男性队列精液中疱疹病毒的流行情况及其与精液参数改变的关联。

设计

一项前瞻性随机研究。

地点

医学院和体外受精诊所。

患者

172名接受常规精液分析的男性患者：80名精液参数正常（对照组），92名精液参数异常。

干预措施

通过手淫采集精液样本。

主要观察指标

疱疹病毒科（单纯疱疹病毒1型[HSV-1]、单纯疱疹病毒2型[HSV-2]、水痘带状疱疹病毒[VZV]、爱泼斯坦-巴尔病毒[EBV]、巨细胞病毒[CMV]、人类疱疹病毒6型[HHV-6]、人类疱疹病毒7型[HHV-7]）的DNA及常规精液参数。

结果

在172份样本中的143份（83.1%）检测到至少一种疱疹病毒的病毒DNA：正常精液样本中HSV-1为2.5%、VZV为1.2%、EBV为45%、CMV为62.5%、HHV-6为70%、HHV-7为0%；异常精液样本中HSV-1为2.1%、VZV为3.2%、EBV为39.1%、CMV为56.5%、HHV-6为66.3%、HHV-7为0%。未发现病毒DNA的存在与精液参数之间存在关联。有趣的是，观察到白细胞精子症与EBV DNA的存在之间具有统计学意义。