Lee Hea Yeon, Lee Bong Kuk, Park Jong Wan, Jung Ho Sup, Kim Jong Min, Kawai Tomoji
The Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan.
Ultramicroscopy. 2008 Sep;108(10):1325-7. doi: 10.1016/j.ultramic.2008.04.089. Epub 2008 May 16.
We report the self-assembly immobilization of functional lipid vesicles (FLVs) by electrostatic interaction onto N-inscription-nanosized geometrics. The well-organized three-dimensional physical structures of liposome were observed by AFM. Generally, two involved forces for the binding to surfaces and the repulsion between individual liposome are necessary to array lipid vesicles individually similar to the physical configuration in solution. The immobilized FLVs demonstrated clearly defined redox activity in electrochemical measurements. We observed a notable current decrease, indicating the binding of the capture antibody with the target human serum albumin (HSA) antigen. We believe these findings can be related to various vesicles applications such as drug delivery system, nanobiosensors and nano-scale membrane function studies.
我们报道了通过静电相互作用将功能性脂质囊泡（FLV）自组装固定在N-转录纳米几何结构上。通过原子力显微镜（AFM）观察到脂质体有序的三维物理结构。一般来说，为了使脂质囊泡像在溶液中的物理构型那样单独排列，需要两种涉及的力，即与表面结合的力和单个脂质体之间的排斥力。固定化的FLV在电化学测量中表现出明确的氧化还原活性。我们观察到电流显著下降，表明捕获抗体与目标人血清白蛋白（HSA）抗原结合。我们相信这些发现可能与各种囊泡应用有关，如药物递送系统、纳米生物传感器和纳米级膜功能研究。
